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CRISPR-Cas9 has emerged as a transformative tool in biotechnology, revolutionizing genetic modification with its precision and versatility. Originating from bacterial immune systems, CRISPR-Cas9 allows targeted modifications to genomic DNA, enabling applications ranging from gene editing in biomedical research to enhancing agricultural traits. This article explores the evolution, mechanisms, applications, implications, and future prospects of CRISPR-Cas9, highlighting its pivotal role in advancing biotechnological innovation.