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Bioinformatics has revolutionized genomic and proteomic research in biotechnology by integrating computational tools with biological sciences. This article explores the transformative role of bioinformatics in deciphering complex biological data, advancing biotechnological applications, and shaping personalized medicine and sustainable bioproduction. Key topics include genomic sequence analysis, comparative genomics, metagenomics, proteomics, protein structure prediction, and applications in precision medicine, biopharmaceutical development, synthetic biology, and agricultural biotechnology. Challenges in data handling, interdisciplinary collaboration, and ethical considerations are also discussed. Bioinformatics continues to drive innovations that enhance our understanding of biological systems and accelerate the development of novel biotechnological solutions.